89KE OPTIONS

We noticed that MobA89K has an N-terminal relaxase domain but lacks a C-terminal helicase area, which commonly possesses a processive five′ to 3′ unwinding action that provides the motive power for strand transfer (Llosa et alwas linearized that has a restriction endonuclease (ScaI or NdeI) after which co-incubated With all the protein factors